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Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel

机译:大孔水凝胶的弹性变形分离亲和捕获的生物颗粒

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摘要

Adsorption of bioparticles to affinity surfaces involves polyvalent interactions, complicating greatly the recovery of the adsorbed material. A unique system for the efficient binding and release of different cells and particles is described. Affinity-bound bioparticles and synthetic particles are detached from the macroporous hydrogel matrix, a so-called cryogel, when the cryogel undergoes elastic deformation. The particle detachment upon elastic deformation is believed to be due to breaking of many of the multipoint attachments between the particles and the affinity matrix and the change in the distance between affinity ligands when the matrix is deformed. However, no release of affinity-bound protein occurred upon elastic deformation. The phenomenon of particle detachment upon elastic deformation is believed to be of a generic nature, because it was demonstrated for a variety of bioparticles of different sizes and for synthetic particles, for different ligand–receptor pairs (IgG–protein A, sugar–ConA, metal ion–chelating ligand), and when the deformation was caused by either external forces (mechanical deformation) or internal forces (the shrinkage of thermosensitive, macroporous hydrogel upon an increase in temperature). The elasticity of cryogel monoliths ensures high recovery of captured cells under mild conditions, with highly retained viability. This property, along with their continuous porous structure makes cryogel monoliths very attractive for applications in affinity cell separation.
机译:生物颗粒在亲和表面的吸附涉及多价相互作用,这大大增加了吸附材料的回收。描述了有效结合和释放不同细胞和颗粒的独特系统。当冷冻凝胶经历弹性变形时,亲和结合的生物颗粒和合成颗粒从大孔水凝胶基质(所谓的冷冻凝胶)分离。认为弹性变形时的颗粒脱离是由于颗粒和亲和基质之间的许多多点附着的破裂以及基质变形时亲和配体之间的距离的变化。但是,在弹性变形时没有释放亲和力结合蛋白。弹性变形时的颗粒分离现象被认为具有普遍性,因为已针对各种大小不同的生物颗粒和合成颗粒,不同的配体-受体对(IgG-蛋白A,糖-ConA,金属离子螯合配体),以及变形是由外力(机械变形)还是内力(温度升高时热敏大孔水凝胶的收缩)引起的。冷冻凝胶整体材料的弹性可确保在温和条件下高度回收捕获的细胞,并具有高度保留的活力。这种特性及其连续的多孔结构使冷冻凝胶整体材料在亲和细胞分离中的应用非常有吸引力。

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