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Effects of Silica Based Biomaterials on Bone Marrow Derived Cells - Material Aspects of Bone Regeneration

机译:二氧化硅基生物材料对骨髓衍生细胞的影响-骨再生的物质方面

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摘要

Silica based biomaterials, such as melt-derived bioactive glasses and sol-gel glasses, have been used for a long time in bone healing applications because of their ability to form hydroxyapatite and to stimulate stem cell proliferation and differentiation. In this study, bone marrow derived cells were cultured with bioactive glass and sol-gel silica, and seeded into porous polymer composite scaffolds that were then implanted femorally and subcutaneously in rats to monitor their migration inside host tissue. Bone marrow derived cells were also injected intraperitoneally.Transplanted cells migrated to various tissues inside the host, including the lung, liver spleen, thymus and bone marrow. The method of transplantation affected the time frame of cell migration, with intraperitoneal injection being the fastest and femoral implantation the slowest, but not the target tissues of migration. Transplanted donor cells had a limited lifetime in the host and were later eliminated from all tested tissues. Bioactive glass, however, affected the implanted cells negatively. When it was present in the scaffold no donor cells were found in any of the tested host tissues. Bioactive glass S53P4 was found to support both osteoblastic and osteoclastic phenotype of bone marrow derived cells, but it was resistant to the resorbing effect of osteoclastic bone marrow derived cells, showing that bioactive glass is rather dissolved through physicochemical reactions than resorbed by cells. Fast-dissolving silica sol gel in microparticulate form was found to increase collagen formation by bone marrow derived cells, while slow dissolving silica microparticles enhanced their proliferation, suggesting that the dissolution rate of silica controls the response of bone marrow derived cells.
机译:基于二氧化硅的生物材料,例如熔融衍生的生物活性玻璃和溶胶-凝胶玻璃,由于其形成羟基磷灰石并刺激干细胞增殖和分化的能力而长期用于骨愈合应用。在这项研究中,将骨髓来源的细胞与生物活性玻璃和溶胶-凝胶二氧化硅一起培养,并接种到多孔聚合物复合支架中,然后将它们股骨和皮下植入大鼠体内,以监测其在宿主组织内的迁移。腹膜内注射骨髓来源的细胞。移植的细胞迁移到宿主内部的各种组织,包括肺,肝脾,胸腺和骨髓。移植方法影响细胞迁移的时间框架,腹膜内注射最快,而股骨植入最慢,但不是迁移的目标组织。移植的供体细胞在宿主中的寿命有限,后来被从所有测试的组织中清除。然而,生物活性玻璃对植入的细胞有负面影响。当其存在于支架中时,在任何测试的宿主组织中均未发现供体细胞。发现生物活性玻璃S53P4既支持骨髓来源的细胞的成骨细胞表型,又支持破骨细胞表型,但它对破骨细胞来源的骨髓的吸收作用具有抵抗力,表明生物活性玻璃通过理化反应溶解而不是通过细胞吸收。发现以微粒形式的快速溶解的二氧化硅溶胶凝胶增加了骨髓衍生细胞的胶原蛋白形成,而缓慢溶解的二氧化硅微粒增强了它们的增殖,表明二氧化硅的溶解速度控制着骨髓衍生细胞的反应。

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  • 作者

    Wilson Timothy;

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  • 年度 2011
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  • 原文格式 PDF
  • 正文语种 en
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