A submerged membrane bioreactor (sMBR) was developed to ferment toxic lignocellulosic hydrolyzate to ethanol. The sMBR achieved high cell density of Saccharomyces cerevisiae during continuous cultivation of the hydrolyzate by completely retaining all yeast cells inside the sMBR. The performance of the sMBR was evaluated based on the ethanol yield and productivity at the dilution rates 0.2, 0.4, 0.6, and 0.8 h-1 with the increase of dilution rate. Results show that the yeast in the sMBR was able to ferment the wood hydrolyzate even at high dilution rates, attaining a maximum volumetric ethanol productivity of 7.94 ± 0.10 g L-1 h-1 at a dilution rate of 0.8 h-1. Ethanol yields were stable at 0.44 ± 0.02 g g-1 during all the tested dilution rates, and the ethanol productivity increased from 2.16 ± 0.15 to 7.94 ± 0.10 g L-1 h-1. The developed sMBR systems running at high yeast density demonstrates a potential for a rapid and productive ethanol production from wood hydrolyzate.
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机译：开发了一种浸没式膜生物反应器（sMBR）以将有毒的木质纤维素水解产物发酵为乙醇。 sMBR通过将所有酵母细胞完全保留在sMBR内，从而在连续培养水解产物的过程中实现了酿酒酵母的高细胞密度。 sMBR的性能基于稀释率0.2、0.4、0.6和0.8 h-1的乙醇产量和生产率随稀释率的增加而评估。结果显示，即使在高稀释率下，sMBR中的酵母也能够发酵木材水解产物，在0.8 h-1的稀释率下，乙醇的最大体积生产率为7.94±0.10 g L-1 h-1。在所有测试的稀释速率下，乙醇产量稳定在0.44±0.02 g g-1，乙醇生产率从2.16±0.15增加到7.94±0.10 g L-1 h-1。在高酵母密度下运行的已开发sMBR系统证明了从木材水解产物快速生产乙醇的潜力。
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