首页> 外文OA文献 >Molecular identification of three novel herpesviruses found in Australian farmed saltwater crocodiles (Crocodylus porosus) and Australian captive freshwater crocodiles (Crocodylus johnstoni)
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Molecular identification of three novel herpesviruses found in Australian farmed saltwater crocodiles (Crocodylus porosus) and Australian captive freshwater crocodiles (Crocodylus johnstoni)

机译:在澳大利亚养殖的咸水鳄(Crocodylus porosus)和澳大利亚圈养的淡水鳄(Crocodylus johnstoni)中发现的三种新型疱疹病毒的分子鉴定

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摘要

As part of a larger investigation into three emerging disease syndromes highlighted by conjunctivitis and pharyngitis, systemic lymphoid proliferation and encephalitis, and lymphonodular skin infiltrates in farmed saltwater crocodiles (Crocodylus porosus) and one emerging syndrome of systemic lymphoid proliferation in captive freshwater crocodiles (Crocodylus johnstoni), cytopathic effects (CPE), including syncytial cell formation, were observed in primary crocodile cell lines exposed to clarified tissue homogenates from affected crocodiles. Ten cell cultures with CPE were then screened for herpesviruses using two broadly-reactive herpesvirus PCRs. Amplicons were obtained from 9 of 10 cell cultures and were sequenced. Three novel herpesviruses were discovered and the phylogenetic analysis of these viruses showed there was a 63% Bayesian posterior probability value supporting these viruses clustering with the subfamily Alphaherpesvirinae, and 100% posterior probability of clustering with a clade containing the Alphaherpesvirinae and other unassigned reptile herpesviruses. It is proposed that they are named Crocodyline herpesvirus (CrHV) 1, 2 and 3. CrHV1 and 2 were only isolated from saltwater crocodiles and CrHV3 was only isolated from freshwater crocodiles. A duplex PCR was designed that was able to detect these herpesviruses in formalin-fixed paraffin-embedded tissues, a sample type that neither of the broadly-reactive PCRs was able to detect these herpesviruses in. This work describes the isolation, molecular detection and phylogeny of these novel herpesviruses but the association that they have with the emerging disease syndromes requires further investigation.
机译:作为对大型结膜炎和咽炎,系统性淋巴样增生和脑炎以及养殖的咸水鳄(Crocodylus porosus)的淋巴结皮肤浸润和三种圈养的淡水鳄鱼(Crocodylus johnstoni)的全身淋巴结增生综合症的三项新兴疾病综合征进行较大调查的一部分),在暴露于来自受影响的鳄鱼的澄清组织匀浆中的原代鳄鱼细胞系中观察到了细胞病变效应(CPE),包括合胞体细胞形成。然后使用两次广泛反应的疱疹病毒PCR筛选十种带有CPE的细胞培养物中的疱疹病毒。从10个细胞培养物中的9个获得扩增子并进行测序。发现了三种新型疱疹病毒,并且对这些病毒的系统发育分析表明,有63%的贝叶斯后验概率值支持这些病毒与亚科Alphaherpesvirinae聚类,以及100%的后验概率与包含Alphaherpesvirinae和其他未分配的爬行动物疱疹病毒的进化枝聚类。建议将它们命名为鳄形疱疹病毒(CrHV)1、2和3。CrHV1和2仅从盐水鳄中分离,而CrHV3仅从淡水鳄中分离。设计了一种能够在福尔马林固定石蜡包埋的组织中检测到这些疱疹病毒的双工PCR,该样品类型是广泛反应性PCR都无法检测到这些疱疹病毒的一种样品。这项工作描述了分离,分子检测和系统发育这些新型疱疹病毒中,有哪些与新出现的疾病综合征有关,需要进一步研究。

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