Nitrogen fixation by associative cyanobacteria in the Canadian Arctic.

摘要

Atmospheric Nu2082-fixation by cyanobacteria is a key source of newly fixed N in nutrient-poor arctic ecosystems. To further determine the causes of N limitation and predict long-term responses to climate change the controls of biological Nu2082-fixation must be better understood. Using acetylene reduction assays we evaluated the spatial and temporal variation in Nu2082-fixation by associative cyanobacteria in various ecosystem types in both the low and high Canadian Arctic. The direct and indirect effects of soil moisture, plant community functional composition, and bryophyte and lichen abundance on rates of Nu2082-fixation were examined at sites varying in latitude and vegetation type. The linkages between N and C cycling processes in arctic systems were examined through paired measurements of Nu2082-fixation, inorganic soil N with surface greenhouse gas fluxes, including COu2082, Nu2082O and CHu2084. Total growing season Nu2082-fixation input across a low arctic landscape was estimated at 0.68 kg hau207b~yru207b~, which is slightly less than twice the estimated average N input 0.39 kg hau207b~yru207b~ via precipitation. Nu2082-fixation by bryophyte-cyanobacterial associations appear to be very important across the Canadian Arctic. Increasing soil moisture was strongly associated with an increasing presence of bryophytes and increasing bryophyte abundance was a major factor determining higher Nu2082-fixation rates at all sites. Shrubs had a negative effect on bryophyte abundance; competition from vascular plants, potentially through shading, may negatively influence Nu2082-fixation. Soil N status was linked to rates or Nu2082-fixation in both the high and low Arctic indicating that these Nu2082-fixing associations act as important point sources of soil N. Higher rates of nitrification may be associated with warmer and drier vegetation types; however, increasing NOu2083-N availability does not appear to increase rates of denitrification. Loss of N through denitrification was not a significant factor in the N cycle at the high arctic sites examined. We found many factors control both the spatial and temporal variability of Nu2082-fixation, including topography, microtopography, vegetation characteristics, microclimatic conditions, nifH abundance and availability of other nutrients, such as phosphorus. Moisture, however, appears to be a key factor not only in determining Nu2082-fixation but also by influencing related nutrient cycling processes.