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A comparison of leaf and petal senescence in wallflower reveals common and distinct patterns of gene expression and physiology

机译:比较壁花中叶片和花瓣的衰老,揭示了基因表达和生理的共同和不同模式

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摘要

Petals and leaves share common evolutionary origins but perform very different functions. However, few studies have compared leaf and petal senescence within the same species. Wallflower (Erysimum linifolium), an ornamental species closely related to Arabidopsis (Arabidopsis thaliana), provide a good species in which to study these processes. Physiological parameters were used to define stages of development and senescence in leaves and petals and to align these stages in the two organs. Treatment with silver thiosulfate confirmed that petal senescence in wallflower is ethylene dependent, and treatment with exogenous cytokinin and 6-methyl purine, an inhibitor of cytokinin oxidase, suggests a role for cytokinins in this process. Subtractive libraries were created, enriched for wallflower genes whose expression is up-regulated during leaf or petal senescence, and used to create a microarray, together with 91 senescence-related Arabidopsis probes. Several microarray hybridization classes were observed demonstrating similarities and differences in gene expression profiles of these two organs. Putative functions were ascribed to 170 sequenced DNA fragments from the libraries. Notable similarities between leaf and petal senescence include a large proportion of remobilization-related genes, such as the cysteine protease gene SENESCENCE-ASSOCIATED GENE12 that was up-regulated in both tissues with age. Interesting differences included the up-regulation of chitinase and glutathione S-transferase genes in senescing petals while their expression remained constant or fell with age in leaves. Semiquantitative reverse transcription-polymerase chain reaction of selected genes from the suppression subtractive hybridization libraries revealed more complex patterns of expression compared with the array data. © 2008 American Society of Plant Biologists.
机译:花瓣和叶子具有共同的进化起源,但执行的功能却截然不同。但是,很少有研究比较同一物种内的叶片和花瓣衰老。壁花(Erysimum linifolium)是与拟南芥(Arabidopsis thaliana)密切相关的观赏物种,是研究这些过程的良好物种。使用生理参数来定义叶片和花瓣的发育和衰老阶段,并在两个器官中对齐这些阶段。用硫代硫酸银处理证实了壁花中的花瓣衰老是乙烯依赖性的,用外源细胞分裂素和细胞分裂素氧化酶抑制剂6-甲基嘌呤处理表明细胞分裂素在此过程中发挥了作用。创建了减法文库,富集了在叶片或花瓣衰老过程中表达上调的壁花基因,并与91个衰老相关的拟南芥探针一起用于创建微阵列。观察到几种微阵列杂交类别,表明这两个器官的基因表达谱具有相似性和差异性。推定的功能归因于文库中170个测序的DNA片段。叶片和花瓣衰老之间的显着相似之处包括大量与动员相关的基因,例如半胱氨酸蛋白酶基因SENESCENCE-ASSOCIATED GENE12在两个组织中均随着年龄的增长而上调。有趣的差异包括衰老花瓣中几丁质酶和谷胱甘肽S-转移酶基因的上调,而它们的表达在叶片中保持恒定或随年龄下降。抑制消减杂交文库中选定基因的半定量逆转录-聚合酶链反应显示与阵列数据相比更复杂的表达模式。 ©2008美国植物生物学家学会。

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