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On-line coupled reversed phase liquid chromatography and gas chromatography: A new sealing design for the TOTAD interface

机译:在线耦合反相液相色谱和气相色谱:TOTAD接口的新型密封设计

摘要

Total elimination of the eluent resulting from the pre-separation step is the critical point when coupling LC to GC. As a helium flow is applied during transfer to eliminate the solvent, the interface used for linking the two chromatographic systems must be properly sealed to prevent gas leaks and to achieve an effective evaporation of the eluent. The aim of this work was to improve the performance of the Through Oven Transfer Adsorption Desorption (TOTAD) interface to remove the eluent coming from LC by modifying the way in which the injector sealing system is held in place. As with the original design, the new approach makes it possible to transfer high volumes at a high rate, but the proposed modification also simplifies the experimental work because the displacement risk of the sealing system is reduced. Analyses of an ester mixture by RPLC-GC were performed to confirm the applicability of the system modification. In this work, volumes of up to 5 ml, at flow rates as high as 2 ml/min, were transferred from LC to GC with almost complete solvent removal even when working in reversed phase mode in the LC step. © 2013 Published by Elsevier B.V.
机译:将液相色谱与气相色谱联用时,从预分离步骤中完全消除洗脱液是关键点。由于在转移过程中施加了氦气流以消除溶剂,因此用于连接两个色谱系统的界面必须适当密封,以防止气体泄漏并实现洗脱液的有效蒸发。这项工作的目的是通过修改进样器密封系统的固定方式来提高直通炉吸附脱附(TOTAD)界面的性能,以去除来自LC的洗脱液。与原始设计一样,新方法使以高速率传输大体积成为可能,但由于降低了密封系统的移位风险,因此所提出的修改方案也简化了实验工作。通过RPLC-GC进行酯混合物的分析,以确认系统修改的适用性。在这项工作中,即使在液相色谱步骤中以反相模式工作时,流速高达2 ml / min的高达5 ml的体积也已从LC转移到GC,几乎可以完全去除溶剂。 ©2013由Elsevier B.V.发布

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