首页> 外文OA文献 >Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC
【2h】

Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC

机译:结合松弛酶TrwC对转移DNA起源的识别和处理

摘要

Relaxases are DNA strand transferases that catalyze the initial and final stages of DNA processing during conjugative cell-to-cell DNA transfer. Upon binding to the origin of transfer (oriT) DNA, relaxase TrwC melts the double helix. The three-dimensional structure of the relaxase domain of TrwC in complex with its cognate DNA at oriT shows a fold built on a two-layer α/β sandwich, with a deep narrow cleft that houses the active site. The DNA includes one arm of an extruded cruciform, an essential feature for specific recognition. This arm is firmly embraced by the protein through a β-ribbon positioned in the DNA major groove and a loop occupying the minor groove. It is followed by a single-stranded DNA segment that enters the active site, after a sharp U-turn forming a hydrophobic cage that traps the N-terminal methionine. Structural analysis combined with site-directed mutagenesis defines the architecture of the active site.
机译:松弛酶是DNA链转移酶,其在结合的细胞间DNA转移过程中催化DNA加工的初始和最终阶段。结合到转移起点(oriT)DNA后,松弛酶TrwC融化了双螺旋。 TrwC的松弛酶结构域的三维结构与其在oriT的同源DNA形成复合体,显示在两层α/β夹心结构上形成的折叠,具有一个深窄的裂缝,该裂缝容纳了活性位点。 DNA包括一个挤压的十字形臂,这是特异性识别的基本特征。该臂通过位于DNA大槽中的β色带和占据小槽的环牢固地被蛋白质包围。紧随其后的是一个单链DNA片段,该片段在急剧的U型转弯后形成一个疏水性笼子,该笼子捕获了N端甲硫氨酸,从而进入了活性位点。结构分析与定点诱变相结合,定义了活性位点的结构。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号