首页> 外文OA文献 >An evolutionarily conserved three-dimensional structure in the vertebrate Irx clusters facilitates enhancer sharing and coregulation
【2h】

An evolutionarily conserved three-dimensional structure in the vertebrate Irx clusters facilitates enhancer sharing and coregulation

机译:脊椎动物Irx簇中进化保守的三维结构有助于增强子共享和共调节

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Developmental gene clusters are paradigms for the study of gene regulation; however, the mechanisms that mediate phenomena such as coregulation and enhancer sharing remain largely elusive. Here we address this issue by analysing the vertebrate Irx clusters. We first present a deep enhancer screen of a 2-Mbp span covering the IrxA cluster. Using chromosome conformation capture, we show that enhancer sharing is widespread within the cluster, explaining its evolutionarily conserved organization. We also identify a three-dimensional architecture, probably formed through interactions with CCCTC-binding factor, which is present within both Irx clusters of mouse, Xenopus and zebrafish. This architecture brings the promoters of the first two genes together in the same chromatin landscape. We propose that this unique and evolutionarily conserved genomic architecture of the vertebrate Irx clusters is essential for the coregulation of the first two genes and simultaneously maintains the third gene in a partially independent regulatory landscape.
机译:发育基因簇是研究基因调控的范例。但是,介导诸如调配和增强子共享等现象的机制仍然很难捉摸。在这里,我们通过分析脊椎动物的Irx簇来解决这个问题。我们首先介绍一个覆盖IrxA簇的2-Mbp跨度的深层增强器屏幕。使用染色体构象捕获,我们表明增强子共享在群集内广泛存在,解释了其进化上保守的组织。我们还确定了三维结构,可能是通过与CCCTC结合因子相互作用而形成的,该结构存在于小鼠,爪蟾和斑马鱼的Irx簇中。这种结构将前两个基因的启动子聚集在相同的染色质景观中。我们提出,脊椎动物Irx簇的这种独特且在进化上保守的基因组结构对于前两个基因的共调是必不可少的,并且同时将第三个基因维持在部分独立的调控环境中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号