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Into the wild of long non-coding RNA in gastrointestinal stromal tumors (GISTs) to explore new prognostic/predictive biomarkers

机译:引入胃肠道间质瘤(GIST)中的长非编码RNA的野生成分,以探索新的预后/预测生物标志物

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摘要

Background: Long Non-coding RNAs (lncRNA) are emerging as essential regulators of genetic and epigenetic networks, andudtheir deregulation may underlie complex diseases, such as carcinogenesis. Several studies have described lncRNAs alterations inudpatients with solid tumors. In particular, in Gastrointestinal Stromal Tumors (GIST), upregulation of HOTAIR has beenudassociated with aggressiveness, metastasis, and poor patients’ survival. In order to gain more detailed insight on the molecularudrole of lncRNAs in GIST, we analyzed in vivo the expression levels of lncRNAs H19 and MALAT1 in surgically resectedudpatients.udMaterial and Methods: The expression of the lnc-RNAs H19 and MALAT1 was evaluated in primary tumor tissue from 20udGIST patients undergoing surgical resection, and paired normal mucosa samples, using quantitative real-time reverseudtranscriptase qRT-PCR. The result was considered reliable if the tumor tissue harboured at least 70% of cancer cells.udResults: H19 was evaluable in 20 patients, MALAT 1 in 8 patients. H19 was overexpressed in 66% (12/20) cancer tissue fromudGIST patients, and the difference of expression between the two groups (tumor tissue vs normal tissue) was found to beudstatistically significant (P= 0.0496). MALAT1 was overexpressed also in 100% (8/8) cancer tissue from GIST patients.udConclusions: H19 and MALAT1 appear frequently upregulated in GIST patients. Further analyses are needed to confirm theseuddata, and evaluate the potential role of such lncRNAs, as prognostic/predictive biomarkers.
机译:背景:长非编码RNA(lncRNA)逐渐成为遗传和表观遗传网络的重要调节剂,其失调可能是诸如致癌等复杂疾病的基础。几项研究描述了实体瘤患者中lncRNA的改变。特别是在胃肠道间质瘤(GIST)中,HOTAIR的上调与侵略性,转移和患者生存不良有关。为了更详细地了解GIST中lncRNA的分子核糖体,我们在体内分析了手术切除的 ud患者中lncRNA H19和MALAT1的表达水平。使用定量实时逆转录酶/逆转录酶qRT-PCR对20例接受手术切除的 udGIST患者的原发肿瘤组织和成对的正常粘膜样本进行了评估。如果肿瘤组织中至少含有70%的癌细胞,则该结果被认为是可靠的。 ud结果:H19在20例患者中可评估,MALAT 1在8例患者中可评估。在来自 udGIST患者的66%(12/20)癌组织中H19过表达,并且发现两组之间的表达差异(肿瘤组织与正常组织)具有统计学差异(P = 0.0496)。 MALAT1在100%(8/8)的GIST患者癌症组织中也过表达。 ud结论:H19和MALAT1在GIST患者中似乎经常上调。需要进一步的分析以确认这些 uddata,并评估此类lncRNA作为预后/预测性生物标志物的潜在作用。

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