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Mutation in a light-regulated glucan synthase-like gene (gsl12) displays light hyper-responsive and callose deficient phenotypes in arabidopsis

机译:光调节的葡聚糖合酶样基因(gsl12)中的突变显示拟南芥中的光高反应性和call质缺乏表型。

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摘要

Light is a very important factor affecting every aspect of plant development.Plant developmental responses to light are sensitive to the direction, intensity, color, andduration of light. Light is perceived by an extensive set of photoreceptors that includesthe red/far-red light?absorbing phytochromes and blue/UV-A light?absorbingcryptochromes. The Arabidopsis mutant seedling hyper-responsive to light 6 (shl6) hasexaggerated developmental responses to available light. In the low light, shl6 seedlingshave a phenotype similar to wild-type plants grown in high light, with short hypocotyls,expanded cotyledons, and well-developed first true leaves. In addition, the roots of shl6are short and highly branched. The SHL6 gene was mapped to a position on chromosome5 between simple sequence length polymorphism (SSLP) markers nga249 and nga151.Two cosmid clones from this interval (introduced by Agrobacterium-mediatedtransformation) complemented the shl6 mutant phenotype. One candidate gene identifiedby complementation is a member of the glycosyltransferase family. The sequence of shl6mutant differs from wild type Columbia allele of this gene (At5g13000) by a singlenucleotide substitution in the first exon. This putative SHL6 gene encodes a member of aglycan synthase-like (GSL12) gene family that includes callose synthase. The ?-1,3-D-glucan callose is found in the cell plate of dividing cells, in pollen mother cell walls, andpollen tubes. Callose synthase and related genes have not been previously implicated indevelopmental responses to light. We also observed that 90% of Col-0 anthers showedhigh callose deposition, but shl6 mutant did not display callose deposition in the anthers.The pollen viability in the shl6 was lower than Col-0. The epidermal cell elongation inshl6 hypocotyls was reduced when compared with Col-0. Therefore, we conclude thatthe mutation in light-regulated SHL6/GSL12 was involved in the synthesis of callose aswell as light signaling.
机译:光是影响植物发育各个方面的重要因素。植物对光的发育反应对光的方向,强度,颜色和持续时间很敏感。光被广泛的感光器感知,包括红色/远红色光吸收性植物色素和蓝色/ UV-A光吸收性隐色素。对光6(shl6)高度敏感的拟南芥突变体幼苗夸大了对可用光的发育反应。在弱光下,shl6幼苗的表型类似于在强光下生长的野生型植物,具有短的下胚轴,膨大的子叶和成熟的第一片真叶。另外,shl6的根短而高度分支。 SHL6基因被定位到5号染色体上的简单序列长度多态性(SSLP)标记nga249和nga151之间的位置。此间隔内的两个粘粒克隆(由农杆菌介导的转化引入)补充了shl6突变表型。通过互补鉴定的一种候选基因是糖基转移酶家族的成员。 shl6突变体的序列与该基因的野生型哥伦比亚等位基因(At5g13000)的不同之处在于第一个外显子中的单核苷酸取代。该推定的SHL6基因编码一个包括糖ose合成酶的糖苷合成酶样(GSL12)基因家族成员。在分裂细胞的细胞板,花粉母细胞壁和花粉管中发现了β-1,3-D-葡聚糖call。愈伤组织合酶和相关基因以前没有涉及光的发育反应。我们还观察到90%的Col-0花药显示出较高的ose实沉积,但shl6突变体在花药中未显示出ose质沉积.shl6中的花粉活力低于Col-0。与Col-0相比,表皮细胞伸长inshl6下胚轴降低了。因此,我们得出结论,受光调节的SHL6 / GSL12中的突变参与了ose的合成以及光信号传导。

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    Byun Bohyun;

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  • 年度 2009
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