Figure 2: Slime mold data generation sequence including (A) real-world aerial map with selected intersections of major roadways (red circles), (B) printed 3D map with topographic features and fixed locations (black dots), (C) representative 3D map with inoculated oat flakes at fixed locations, (D) resulting slime mold network (yellow threads) after 3-day incubation, (E) ImageJ software visualizing network connections, and (F) example length measurement of both freeform and direct distance between nodes using ImageJ tool.