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Spectroscopic photoacoustic imaging of cervical tissue composition in excised human samples

机译:切除的人样品中宫颈组织组成的光谱光声成像

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摘要

ObjectiveCervical remodeling is an important component in determining the pathway of parturition; therefore, assessing changes in cervical tissue composition may provide information about the cervix's status beyond the measurement of cervical length. Photoacoustic imaging is a non-invasive ultrasound-based technology that captures acoustic signals emitted by tissue components in response to laser pulses. This optical information allows for the determination of the collagen-to-water ratio (CWR). The purpose of this study was to compare the CWR evaluated by using spectroscopic photoacoustic (sPA) imaging in cervical samples obtained from pregnant and non-pregnant women.MethodsThis cross-sectional study comprised cervical biopsies obtained at the time of hysterectomy (n = 8) and at the scheduled cesarean delivery in pregnant women at term who were not in labor (n = 8). The cervical CWR was analyzed using a fiber-optic light-delivery system integrated to an ultrasound probe. The photoacoustic signals were acquired within the range of wavelengths that cover the peak absorption of collagen and water. Differences in the CWR between cervical samples from pregnant and non-pregnant women were analyzed. Hematoxylin and eosin and Sirius Red stains were used to compare the collagen content of cervical samples in these two groups.ResultsEight cervix samples were obtained after hysterectomy, four from women ≤41 years of age and four from women ≥43 years of age; all cervical samples (n = 8) from pregnant women were obtained after 37 weeks of gestation at the time of cesarean section. The average CWR in cervical tissue samples from pregnant women was 18.7% (SD 7.5%), while in samples from non-pregnant women, it was 55.0% (SD 20.3%). There was a significantly higher CWR in the non-pregnant group compared to the pregnant group with a p-value <0.001. A subgroup analysis that compared the CWR in cervical samples from pregnant women and non-pregnant women ≤41 years of age (mean 46.3%, SD 23.1%) also showed a significantly higher CWR (p <0.01). Lower collagen content in the pregnancy group was confirmed by histological analysis, which revealed the loss of tissue composition, increased water content, and collagen degradation.ConclusionThe proposed bimodal ultrasound and sPA imaging system can provide information on the biochemical composition of cervical tissue in pregnant and non-pregnant women. Photoacoustic imaging showed a higher collagen content in cervical samples from non-pregnant women as compared to those from pregnant women, which matched with the histological analysis. This novel imaging method envisions a new potential for a sensitive diagnostic tool in the evaluation of cervical tissue composition.
机译:客观感应重塑是确定分娩途径的重要组成部分;因此,评估宫颈组织组合物的变化可以提供有关宫颈状况超出宫颈长度的测量的信息。光声成像是一种基于非侵入性超声的技术,响应于激光脉冲而捕获由组织成分发出的声学信号。该光学信息允许确定胶原 - 水比(CWR)。本研究的目的是将通过在孕妇和非孕妇中获得的宫颈样品中使用光谱光声(SPA)成像进行比较CWR.THISSTHIS横截面研究,该研究包括在子宫切除术时获得的宫颈活组织检查(n = 8)在孕妇的预定剖腹产中,孕妇不在劳动(n = 8)。使用集成到超声探头的光纤光输送系统分析宫颈CWR。在覆盖胶原和水的峰值吸收的波长范围内获得光声信号。分析了患有孕妇和非孕妇颈椎样品之间的差异。苏木精和曙红和天狼星红污渍用于比较这两组宫颈样品的胶原蛋白含量。患有子宫颈样品在子宫切除术后获得,女性≤41岁以下,来自女性≥43岁。在剖宫产时妊娠37周后,获得来自孕妇的所有颈椎样品(n = 8)。孕妇的宫颈组织样品中的平均CWR为18.7%(SD 7.5%),而在来自非孕妇的样品中,它是55.0%(SD 20.3%)。与P值<0.001的怀孕组相比,非妊娠组中有一个显着更高的CWR。亚组分析,将患有孕妇的宫颈样品中的CWR与非孕妇≤41岁(平均46.3%,SD 23.1%)表现出明显更高的CWR(P <0.01)。通过组织学分析证实了怀孕组中的较低胶原蛋白含量,揭示了组织成分的损失,增加的水含量和胶原降解。结论所提出的双峰超声和SPA成像系统可以提供有关孕妇宫颈组织的生化组成的信息非孕妇。与来自组织学分析相匹配的孕妇相比,光声成像在非孕妇的宫颈样品中显示出更高的胶原蛋白含量。这种新型成像方法设想在评估宫颈组织组合物中的敏感诊断工具的新电位。

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