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Evaluation of an FIA Operated Amperometric Bacterial Biosensor, Based on Pseudomonas Putida F1 for the Detection of Benzene, Toluene, Ethylbenzene, and Xylenes (BTEX)

机译:基于恶臭假单胞菌F1的FIA操作的安培菌生物传感器的评估,用于检测苯,甲苯,乙苯和二甲苯(BTEX)

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摘要

Recently, the development and optimization of a flow injection analysis (FIA) operated bacterial biosensor based on the aerobic catabolism of Pseudomonas putida ML2 was reported in the literature (Lanyon et al. 2004, 2005). By adapting information from these reports, we investigated whether operating parameters and procedures of the benzene biosensor could be directly applied to a new system based on a different bacterial strain for the detection of the whole benzene, toluene, ethylbenzene, and xylenes range. Cells of the investigated bacterial strain, Pseudomonas putida F1, were immobilized between two cellulose acetate membranes and fixed onto a Clark dissolved oxygen electrode. The P. putida F1 aerobically degrades benzene, toluene, and ethylbenzene (BTE) (Cho et al. 2000). The BTE biosensor in kinetic mode FIA displayed a linear range of 0.02-0.14 mM benzene (response time: 5 min, base-line recovery time: 15 min), 0.05-0.2 mM toluene (response time: 8 min, baseline recovery time: 20 min), and 0.1-0.2 mM ethylbenzene (response time: 12 min, baseline recovery time: 30 min), respectively. Due to the differences in sensitivity, response, and baseline recovery times for BTE, it was possible to differentiate each compound in mixtures of these volatile organic compounds (VOCs). No response for xylenes could be obtained since they cannot be completely metabolized by this bacterial strain. However, it was reported that the range of compounds degradable by P. putida F1 can possibly be expanded by cultivating the cells on different carbon sources (Choi et al. 2003). The sensor showed good intra- and interassay reproducibility, and all obtained results were comparable with those reported in the literature. The demonstrated reproducibility and the simplicity and ease of use as well as the portability for in situ measurements indicates that the biosensor could be suitable as a reliable initial warning device for elevated BTE levels in indoor and outdoor environments.
机译:最近,文献报道了基于恶臭假单胞菌ML2的需氧分解代谢的流动注射分析(FIA)操作的细菌生物传感器的开发和优化(Lanyon等人,2004,2005)。通过利用这些报告中的信息,我们调查了苯生物传感器的操作参数和程序是否可以直接应用于基于不同细菌菌株的新系统,以检测整个苯,甲苯,乙苯和二甲苯的范围。将研究的细菌菌株恶臭假单胞菌F1的细胞固定在两个醋酸纤维素膜之间,并固定在Clark溶解氧电极上。恶臭假单胞菌F1有氧降解苯,甲苯和乙苯(Bho)(Cho et al。2000)。动力学模式FIA中的BTE生物传感器显示线性范围为0.02-0.14 mM苯(响应时间:5分钟,基线恢复时间:15分钟),0.05-0.2 mM甲苯(响应时间:8分钟,基线恢复时间: 20分钟)和0.1-0.2 mM乙苯(响应时间:12分钟,基线恢复时间:30分钟)。由于BTE的灵敏度,响应时间和基线恢复时间不同,因此可以区分这些挥发性有机化合物(VOC)混合物中的每种化合物。由于二甲苯不能被该细菌菌株完全代谢,因此无法获得对二甲苯的反应。但是,据报道,恶臭假单胞菌F1可降解的化合物的范围可能可以通过在不同碳源上培养细胞来扩大(Choi等,2003)。该传感器显示出良好的批内和批间重复性,所有获得的结果均与文献报道的结果相当。所展示的可再现性,简单易用以及现场测量的便携性表明,该生物传感器可能适合作为室内和室外环境中升高的BTE水平的可靠初始警告设备。

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