Preparation and Monitoring of Lead Acetate Containing Drinking Water Solutionsfor Toxicity Studies

摘要

The protocols developed and implemented to provide lead acetate containingdrinking water solutions for animal toxicity studies are described in detail. The procedure involved preparation of 20 liter batches of high concentration lead acetate solution (20,000 ppm) at National Institute of Standards and Technology (NIST). Conventional flame atomic absorption spectrophotometry (FAAS) was employed to confirm that the lead concentration of the solution was within five percent of the target value. This solution was then dispensed into containers of appropriate volume such that upon dilution at the animal research facility, drinking water solutions containing lead concentrations of 50, 250 and 1000 ppm were produced. Samples of these diluted solutions and samples taken from animal cage water bottles were returned to NIST for follow-up FAAS determination of lead concentration to insure that animal exposure to lead was meeting experimental goals.