Research and Development of Adenovirus Vaccines and Parainfluenza Virus Vaccines and Adaptation of Respiratory Syncytial Virus

摘要

Adenovirus vaccine studies have included, (1) long term antigenic stability studies of vaccines prepared with types 1, 2 and 5, both aqueous, alum and lyophilized formulations; (2) karyology of WI-38 at vaccine passage levels; (3) electron microscope studies of soluble antigen vaccine components; (4) examination of all vaccine passage level seed for AAV contamination; (5) initiation of a small clinical trial in human adults utilizing type 5 vaccines; (6) evaluation of small laboratory preparations of adenovirus types 3, 4 and 7 monovalent vaccines utilizing methodology worked out for types 1, 2 and 5; (7) development of new disruption methodology for types 3, 4 and 7; and (8) the preparation of two pilot lots of adenovirus type 5 vaccine used in the initiation of DNA tracer studies which are incomplete at this time. Parainfluenza 1 and 2 were propagated in embryonated eggs and amniotic fluids were collected for the preparation of future vaccines. Stability studies on parainfluenza 1 virus vaccine containing 5% lactose + 0.5% HSA and influenza buffer stored at 4 deg C and 37 deg C are being followed by means of HA tests. (Author)