Immobilization of Whole Cells Containing Glucose Isomerase.

摘要

Various procedures for construction of secondary cellulose acetate membranes with entrapped whole cells (containing glucose isomerase) have been developed and studied. Different effects on the behavior of the constructed membranes were noted and reported. Several methods of preparing the casting mixture for membrane construction and the effects of the composition of the casting mixture were studied. Membranes were also constructed by using different solvent systems,secondary cellulose acetates,and casting media. The numerous membranes constructed were compared in terms of their observed activities with equivalent amounts of non-immobilized whole cells. The results established that the best secondary cellulose acetate membrane preparation recipe is to use methylene chloride-acetone (6:1) as solvent with a loading ratio of cellulose acetate to whole cells of 2:1for the casting mixture. Membranes should be cast on glass and air dried.