A Reagentless Solid Surface Monitoring System for Measuring the Frequency of Dialysis.

摘要

Highly selective,ultra-sensitive fluorometric methods were developed for the assay of tryptophan and its metabolites, 5-hydroxytryptophan and serotonin,for the enzymes transketolase and CPK (creatine phosphokinase) and for various substances to indicate the efficiency of dialysis-uric acid,creatine,histidine and urea. A reagentless solid surface monitoring system was used for analysis. In this system all the reagents for analysis are placed on the surface of a silicone rubber pad. Upon addition of the clinical sample a change in fluorescence is observed,proportional to the concentration of the substance being measured.