Metal-Induced Alteration of the Cell Membrane/Cytoplasm Complex Studied by Flow Cytometry and Detergent Lysis

摘要

Flow cytometric analysis of the cell cycle is most effectively accomplished with membrane-/cytoplasma-free (clean) nuclei. Non-ionic detergents (e.g. NP40 or Triton X-100) commonly are employed to solubilize cells membranes/cytoplasm to produce 'clean' nuclei. Treatment of murine erythroleukemic cells with tri-n-butylin methoxide, cadmium acetate, zinc sulfate, or lead acetate alters the properties of the cell membrane/cytoplasm complex making it resistant to NP40 dissolution. On a molar basis, the organotin compound was more effective in inducing resistance to detergent-mediated dissolution than the inorganic metal compounds. Resistance to NO40-mediated dissolution was manifested as an increase in the flow cytometric parameters 90 degree scatter and fluorescein isothiocyanate fluorescence and was confirmed by light microscopy. (Copyright (c) Elsevier Scientific Publishers Ireland, Ltd.)