Isolation and Characterization of B-Glucosidase Gene and B-Glucosidase of Trichoderma Viride. Progress Report

摘要

The goal is to clone and characterize each of the cellulase genes from Trichoderma. This report is principally concerned with B-glucosidase. The induction of the Trichoderma cellulase complex by cellulose and by the soluble inducer, sophorose, has been demonstrated. B-glucosidase has been isolated and purified to homogeneity. The enzyme contains significant amounts of carbohydrate and has a molecular weight greater than bovine serum albumin (68,000). (ERA citation 08:052302)