Spiperone Binding Sites in Rat Primary Cultures, C6 Glioma, and B104 Neuroblastoma ( Exp 3 H)

摘要

Binding sites for ( exp 3 H) spiperone were detected on membranes from primary glial cultures from neonatal rat cortex and striatum and from the C6 glioma cells. ( exp 3 H) spiperone binding was displacable by d-butaclamol. However, competition studies suggest that ( exp 3 H) spiperone binding to primary glial cultures was mainly to serotonergic sites, and binding to the C6 glioma was alpha-adrenergic. No specific binding was detected to membranes from B104 neuroblastoma cells. Although ( exp 3 H) spiperone binding to glial sites in whole striatum under generally used conditions is small (about 10%), the striatal glial hyperactivity which is often associated with neuronal degeneration could lead to an overestimation of presumed neuronal binding sites for dopaminergic ligands. (ERA citation 08:039400)