Synchronous Fluorescence/Matrix Isolation Method for Trace Organic Analysis. Progress Report, July 1980-July 1981

摘要

The first phase of the project involved assembling the instrumentation, constructing the computer interface, writing computer software, and preliminary testing. The principal components of the system are a high intensity Xenon arc lamp for excitation, two flat field f/4 spectrographs (J-4 Model UFS-2000) for dispersion of excitation and emission spectra, fused silica cuvette, an image dissector camera system (EMR Photoelectric 658A) for detection of emission intensity, a custom designed and constructed computer interface for scan control and data acquisition, and PDP-8 computer with assorted peripherals. Preliminary data indicate a range of at least 400 nm can be resolved to about 1 nm for both excitation and emission spectra. Computer interface and software are designed to permit any one of three modes of operation, namely a) the diagonal scans required to generate synchronous fluorescence spectra directly, b) random access to any set of excitation/emission coordinates, and c) total scans of any selected area (up to 100%) of the complete set of excitation/emission coordinates. The complete system became operational in late 1980 and we have been evaluating the system since that time. Data for anthracene are presented here to illustrate system response. (ERA citation 06:035236)