Determine the Yield of Micronucleated Cells in Primary Human Fibroblasts Exposed to Focused Soft X-rays (Final Report)

摘要

This project was a small part of a larger collaborative study headed by Dr Aloke Chatterjee, (Lawrence Berkley National Laboratory) and including Drs Les Braby, John Ford (Texas A&M) and Kathy Held (MGH Boston), which was developing an integrated theoretical and experimental model of the radiation-induced bystander response. Our part of the study has been to determine the effectiveness of soft X-rays at inducing chromosomal damage under conditions of direct and bystander exposure. The aim was to compare this with the effectiveness of the low energy 60 kV electron microbeam available at Texas A&M. Previous studies have been performed with primary human fibroblasts measuring micronuclei formation to determine the relative yields of direct versus bystander mediated micronuclei formation after cells were individually irradiated utilising our novel focussed soft X-ray microprobe, which is capable of producing localised submicron beams of carbon-K (278 eV) X-rays. Only a brief overview is given here as the study has been published in several papers.