Effect of Quaternization of the Glucose Oxidase 'Wiring' Redox Polymer on theMaximum Current Densities of Glucose Electrodes

摘要

Redox polymers based on poly (vinyl pyridine) complex of (Os(bpy)2C1)1(+/2+) werequaternized with methyl iodide and the quaternized polymers were used to wire' glucose oxidase. Quaternization enhanced both the rate of electron transport in crosslinked redox hydrogels containing glucose oxidase and the strength of the electrostatic complex formed between the polycationic redox polymer and the polyanionic glucose oxidase. Quaternization with methyl groups also decreased the number of pyridine rings available for crosslinking by the water soluble crosslinker, poly (ethylene glycol) diglycidyl ether. The current densities of glucose electrooxidation increased with the degree of quaternization of the 'wires' until one third of the pyridine rings were quaternized, and the activation energies decreased until one-half of the rings were quaternized.