Percutaneous Absorption of Volatile Chemicals

摘要

The purpose of this project was to assess the percutaneous absorption of twovolatile organic compounds, chloropentafluorobenzene (CPFB) and dichlorobenzene (DCB) in the isolated perfused porcine skin flap (IPPSF) model. An independent theoretical goal was to begin to develop a mathematical framework to assess vehicle-compound interactions which occur during dermal exposure. Assessment of the percutaneous absorption and penetration of volatile compounds is difficult. The process of studying these compounds involved 5 steps: (1) development of an IPPSF cradle chamber to trap the evaporated compound in the area next to the skin, (2) assessment of the mass of CPFB that was absorbed into the perfusate from CPFB which was evaporated from excised skin, (3) exposure of the IPPSF to neat test compounds and test compounds in a vehicle, (4) assessment of the mass of the test compound in the perfusate is a result of exposure to the volatile compound vapor, and (5) development of a dosing dome that allowed dosing a vapor without vapor uptake directly into the perfusate. Relevant absorption parameters were then determined. These studies demonstrated dose-dependent absorption of CPFB and DCB in skin which was further modulated by concomitant exposure to vehicle. The data obtained could be used as direct input into a systemic risk assessment model.