Optimizing the Immuno-Surface Characteristics for Bio-Sensors and Filters Through Modeling and Experiments

摘要

The scope of this work integrated experimental exploration with model development, and optimization studies for determining the best configuration and composition of immuno-surfaces for biosensors as inspired by the B-cell membrane. One objective of this work was to determine alteration in antigen capture on immuno-surfaces as a function of antibody density and functionality achieved by different protein immobilization methods. Another was to generate a model that reflects multivalent antigen binding dynamics to immobilized antibodies and incorporates the effective far-field antibody surface density, the local antibody surface density once the antibody is bound, and the flexibility and functional range of motion of the immobilized antibodies. The study also designed, synthesized, and evaluated the influence of surface roughness and energetics on immunoassay functionality. Finally, two mathematical models were created to quantitatively evaluate the effects of antibody and epitope diffusion on antigen capture.