Antibody-NKG2D Ligand (Rae-1Beta) Fusion Protein for Breast Cancer Therapy

摘要

Antibody-NKG2D ligand fusion protein targeted against the breast tumor antigen HER2. Using murine breast tumors designed to express the HER2 target antigen and the murine NKG2D ligand Rae-1BETA, we constructed an anti- HER2 IgG3-CH3-Rae-1BETA fusion protein by genetically fusing Rae-1BETA at the 3' end of CH3 domain of anti-HER2 IgG3 antibody. The purified Rae-I(underline) fusion proteins demonstrated ability to bind antigen on tumor cells and NKG2D on NK cells. The antibody-Rae-1(underline) fusion proteins showed increased cytotoxic activity directed against tumor targets.