Evaluation of a Test Article in the L5178Y TK+/-Mouse Lymphoma Mutagenesis Assay with Colony Size Evaluation in the Presence and Absence of Induced Rat Liver S-9 with a Confirmatory Study. Test Article: 3-Nitro-1,2,4- Triazol-5-one (NTO)

摘要

3-Nitro-1,2,4-triazol-5-one (NTO, 99.6% pure) was tested for its potentials to induce mutation at the thymidine kinase locus of L5178Y TK +/- mouse lymphoma cells in vitro with and without metabolic activation according to OECD TG 476 in compliance with Good Laboratory Practice. Cell cultures were treated with NTO at concentrations of 10, 50, 100, 250, 500, 1000, 2500, and 5000 with and without activation and were found negative in the mutation assay. A confirmatory mutation assay with 24 hrs treatment period and without activation was performed by using the same concentrations of NTO. These results were also negative.