Deregulation of miRNAs Contributes to Development and Progression of Prostate Cancer. Annjual Report August 1, 2009-July 31, 2010

摘要

The overall hypothesis of our proposal is that miR-125b acts as an oncogene, contributing to the development and progression of prostate cancer. In this grant, we proposed to test the ability of aberrantly-expressed miR-125b to promote tumorigenesis and to induce AI growth in three specific aims. In the first year, our focusThe overall hypothesis of our proposal is that miR-125b acts as an oncogene, contributing to the development and progression of prostate cancer. In this grant, we proposed to test the ability of aberrantly- expressed miR-125b to promote tumorigenesis and to induce AI growth in three specific aims. In the first year, our focus was on Aim 1: to validate targets of miR-125b in prostate cancer. We have found that i) miR-125b directly targets three key pro-apoptotic molecules: p53, Puma and Bak1, and ii) AR signaling down-regulates p53, Puma and Bak1 via miR-125b in CaP cells. Our results obtained in the first year have provided new insight into the molecular mechanisms related to tumorigenesis and CR growth of CaP.