Hypothermia Following Injection of 2-Deoxy-D-Glucose into Selected Hypothalamic Sites,

摘要

From previous studies with 2-deoxy-D-glucose (2-DG) an inhibitor of glucose utilization,we postulated that the resultant intracellular glucopenia affects central neuronal pathways involved in the control of peripheral heat production. In this investigation we have delineated these thermoregulatory sites by stereotaxically injecting micro-quantities of 2-DG into the hypothalamus of the rat and monitoring core temperature T-re. After stabilization of T-re, 2 microliters of 20micrograms of 2-DG was injected into 350-400 g rats at 23 +or - 1 C. Significant decreases in T-re were noted for the anterior hypothalamic, ventromedial and dorsomedial nuclei as well as the lateral and posterior hypothalamic areas. With the ventral premammillary nucleus (PMV) mean nadir T-re decreases of -1.1 C occurred 1hour after administration of 2-DG,was significantly depressed after 3.1/2hours,and returned to basal values after 4hours. Dose dependant response was observed only for this nucleus. Of a total of 21sites studied in both the anterior and posterior hypothalamus,the PMV,an area of unknown physiological function,was the most sensitive to glucose deprivation.