Microbial Interactions with Several Munitions Compounds: 1,3-Dinitrobenzene, 1,3,5-Trinitrobenzene, and 3,5-Dinitroaniline

摘要

The microbial degradation of 1,3-dinitrobenzene was complete or near complete in Tennesee River water but not in all other environmental watersources tested. Microorganisms from the Tennessee River could utilize 1,3-dinitrobenzene as a sole carbon source for growth and could mineralize the compound. The half-life of 1,3-dinistrobenzene at 25 C and 1,000,000 colony-forming units per milliliter was approximately 1 day in Tennessee River water samples and slightly less than 10 days in the presence of enrichment cultures developed from the samples. The differences in the measured half-lives likely reflects basic microbiological differences in two approaches used to obtain half-lives. Decreases in the concentrations of 1,3,5-trinitrobenzene and 3,5-dinitroaniline mediated by microorganisms in Tennessee River water samples were incomplete and unsustained. Maximum reductions in the concentrations of the compoiunds required the presence of sediments and even then we were slow. Neither compound would serve as a carbon source for microbial growth, but both compounds were metabolized in the presence of added nutrients. The metabolism of the compounds did not lead to their ultimate biodegradation. Nitro group reduction was seen to occur with both 1,3,5-dtrinitrobenzene and 3,5-dinitroaniline in the presence of added nutrients and laboratory cultures of Tennessee River microorganisms and also occurred with 1,3,5-trinitrobenzene in Tennessee River water samples. (Author)