Phase-Resolved Fluorometric Determinations of Four-Component Systems Using Two Modulation Frequencies

摘要

The use of phase-resolved fluorometric measurements at two modulation frequencies and three emission wavelengths to simultaneously determine 1-chloroanthracene (1CA), 9,10-diphenylanthracene (9,10DPA), 9-phenylanthracene (9PA), and 9-vinylanthracene (9VA) in mixtures of these four components is described. Relative errors below 2% were obtained for each of the four components in all the mixtures studied. Relative standard deviations of 1.15%, 3.95%, 1.27%, and 9.23% were obtained for 1CA, 9PA, 9,10DPA, and 9VA, respectively, for a mixture in which the combined fluorescence intensity contribution from 9PA and 9VA (which have a fluorescence lifetime difference of 1.42 ns and very similar emission spectra) was less than 10% of the total fluorescence emission intensity.