In vivo Distribution of 14C Radiolabeled Soman ((3,3-Dimethyl-2-Butoxy)-Methylphosphorylfluoride) in the Central Nervous System of the Rat

摘要

Radiolabeled 14C soman, a potent anticholinesterase, was administered to rats at a dose of 0.75 LD50. Prior to being quick frozen, the animals were held for 2 min, 32 min or 48 h after dosing. Analysis of autoradiographs taken from cryostat sections through the central nervous system (CNS) showed that at 2 and 32 min radiolabel was primarily distributed in the blood, the choroid plexus and the cerebrospinal fluid (CSF), with several brain nuclei showing small increases in labeling density. At 48 h there was marked accumulation of radiolabel in the caudate and accumbens nuclei compared to all other brain areas. The above results showed little similarity between cholinesterase localization and distribution of radiolabel. Keywords: Soman, Distribution, Rats, Autoradiography, Organophosphate, In vivo analysis. (kt)