Fluorescence Lifetime Selectivity in Multifrequency Phase-Resolved Fluorescence Spectroscopy

摘要

Theoretical aspects of the implementation of fluorescence lifetime selectivity in multifrequency phase resolved fluorescence spectroscopy (PRFS) are discussed. The dependence of phase-resolved fluorescence intensities on the dynamic instrumental parameters of excitation modulation frequency and detector phase angle, as well as on fluorescence lifetime, is described. The different types of fluorescence lifetime filtering that can be effectively achieved include long-pass, short-pass, and bandpass functions. The ability to continuously scan both modulation frequency and detector phase angle greatly increases the potential fo PRFS for fluorimetric analysis. Fluorescence, Luminescence; Spectroscopic techniques; Time-resolved spectroscopy; Phase-resolved fluorescence spectroscopy. Reprints. (mjm)