Molecular Biological Studies on the Biogenesis of Human Cholinesterases In vivo and as Directed by Cloned Cholinesterase DNA Sequences.

摘要

In order to study the multileveled regulation of the human cholinesterase genes and their protein products, the cDNAs encoding for butyrylcholinesterase and acetylcholinesterase in several human tissues were isolated and compared to each other and to other cDNAs encoding related proteins. Xenopus oocytes were used to express the synthetic mRNA for butyrylcholinesterase alone or with tissue mRNAs. Cross-homologies and structural differences between cholinesterases were revealed by antibodies against bacterially produced butyrylcholinesterase polypeptides, which also react with the homologous thyroglobulin. In situ hybridization was employed to reveal the expression of cholinesterase mRNA in human oocytes. Amplification of the CHE gene was found in a family exposed to parathion as well as in blood cells from patients with leukemias or platelet disorders.