Early Diagnosis and Treatment of Opportunistic Mycobacterial Infections in HIV-Seropositive AIDS Patients.

摘要

A highly specific Enzyme-Linked Immunosorbent Essay system has been developed to detect circulating antibody in sera raised against the type-specific polar glycopeptidolipid antigens of Mycobacterium avium. These antigenic structures are unique to M. avium, and hence the purpose of the project was to exploit this knowledge to determine the prevalence of such antibodies in homosexual men at increased risk of Human Immunodeficiency Virus infection, and thus subsequent opportunistic infection with disseminated M. avium disease. Progress within the project to date has consisted of extraction of a large number of serotype-specific Glycopeptido Lipid antigens, and conformation of their purity using thin-layer chromatography and Gas Chromatography/Mass Spectrometry. The ELISA system was then initially tested for its specificity using a panel of monoclonal and polyclonal rabbit and mouse anti-GPL antibodies. RA 1, M. avium, In vivo, ELISA, Mycobacterium, Diagnostic serology. (jg)