Genotoxicity Assessment of Perfluorodecanoic Acid Using a Battery of In vitro and In vivo/In vitro Assays.

摘要

Perfluoro-n-decanoic acid (PFDA), a perfluorinated fatty acid was evaluated in in vitro bioassays to assess its potential genotoxic activity. The assays conducted were the Ames Salmonella/microsomal mutagenicity assay, the Chinese hamster ovary (CHO) gene mutation assay, the CHO/sister chromatid exchange (SCE) and chromosome aberrations assays, the BALB/c-3T3 cell transformation assay and in vivo/in vitro unscheduled DNA (UDS) and S-phase synthesis assays. The results of the genetic tests indicated that PFDA does not interact with genetic material except possibly at highly toxic doses. No responses were found in the Ames assay, SCE assay, and cell transformation assay. However, in the chromosomal aberrations assay highly toxic treatments of cells in culture with PFDA induced a dose-related increase in chromosomal aberrations in the presence of S9 microsomal enzymes. PFDA also caused an increase in S-phase DNA synthesis at both 16 and 48 hours after dosing. The maximum response was obtained at a dose of 22 mg/kg. At both higher and lower concentrations the response was decreased. These response indicated the rapid onset of a low-level hepatotoxicity but the genetic test battery results predicted that little or no genetic risk would occur from exposure to PFDA.