Altered Signal Transduction in Renal Cell Injury Following Hemorrhagic Shock orAnoxia

摘要

The principal focus of this project is to characterize the effects of shock andshock-related cell injuries on: (1) cell structure and function; (2) regulation of cell and organelle ion regulation, especially that of (Ca2+)i; (3) the role of (Ca2+)i in the orchestration of transmembrane signalling in shock and cell injury; (4) the role of cytoskeletal membrane interactions in the changes that result; and (5) the effects of ion deregulation following sublethal injury on altered gene expression. Our strategy is to use both anoxia/ischemia animal and human in vitro model systems and an in vitro hemorrhagic shock model developed in our laboratories. With this approach, we hope to be able to extrapolate animal data to man. In addition to the use of routine established morphological and biochemical techniques, we will continue to use newly developed technologies such as digital imaging fluorescence microscopy to measure (Ca2+)i and (H+)i in living cells and molecular biology techniques to study gene expression. (js)