Development of a Therapeutic Agent for Wound-Healing Enhancement

摘要

The enhancement of wound healing using pharmaceutical agents can be of greatbenefit in surgery, burns and dermal ulcers, ophthalmology, and orthopedics, especially for the Navy. the objective of this research is the development of therapeutic agents that can enhance wound healing by stimulating the mitogenesis and chemotaxis of appropriate cells. Platelet-derived growth factor (PDGF) has both these properties and it has been reported that both these properties are located in different domains. Our aim is to identify these active domains. Specifically, we proposed to Synthesize a series of peptides of various lengths from the A and B chain sequences of human PDGF. Proteolytic cleavage sites and computer-graphic sequence analysis were considered in selecting the sequence and length of the peptide to be synthesized. Test the binding of the peptides to mouse 3T3 cells by radioreceptor assay. These cells have receptors for both PDGF A and B chains. Test peptides for mitogenic and chemotactic activity. Test potent peptides for wound healing in a mouse model. Synthesize smaller fragments of active peptides to determine the minimum sequence required for activity.