Biochemical Testing of Potentially Hazardous Chemicals for Toxicity Using Mammalian Liver Cell Cultures.

摘要

This proposal dealt with the testing of selected chemicals for their possible toxic effects on humans through the use of established mouse (HEPA-2) and human (HEP-G2) liver cell lines. The objective of the work was to eliminate the use of long-term more costly animal models to assess chemical toxicity. These established liver cell lines were chosen or use in this project because they are well characterized biochemically, and continue to express many of the normal functions carried out by the liver. The human HEP-G2 cells are known to secrete 17 different major plasma proteins into the culture medium. The HEPA-2 mouse cells are known to synthesize and to secrete albumin, alpha-fetoprotein, transferrin, ceruloplasmin and haptoglobin while being cultured. Most of the proposed work dealt with the activation of specific detoxifying enzymes of the liver that are involved in the metabolism of xenobiotics. On the other hand, not all hepatotoxins induce these enzyme systems while causing various forms of alteration in the hepatocytes. Therefore, alterations in the expression of the liver-specific functions listed above. Biochemical testing; Toxic chemicals; Mammalian liver cells; Tissue-specific functions; HepatotoxicitY; RA 3 Cytotoxicity; Mouse cell line HEPA-2; Human celline HEP-G2.