DNA Lesions in Medaka (O. latipes): Development of a Micro-Method for TissueAnalysis Using Gas Chromatography-Mass Spectrometry

摘要

We have reached a point where substantial reductions have been made in the amountof tissue required for GC-MS analysis. In the future, we will analyze the tissues from a number of normal and exposed organisms using the micro method to hopefully validate the approach. The work on monoclonal antibodies is proceeding favorably. Shortly, we expect to obtain a sufficiently pure 8-OH-adenosine to initiate the antigen phase of the work. The FT-IR effort, if successful, will allow for a rapid assessment of DNA modifications in relation to carcinogenesis using as little as a few micrograms of DNA. Overall, the project is on schedule. Fish, Molecular biology, Environmental assessment, RA III.