Rapid Dispersion of Polymicrobial Wound Biofilms with Depolymerase Enzymes.

摘要

During the award period, we successfully developed protocols to extract biofilm EPS associated with ESKAPE organisms (Enterococcus, Staphylococcus, Klebsiella, Acinetobacter, Pseudomonas, and Enterobacter species). We further performed composition and linkage analysis on these samples and determined that 2 and 3-linked mannose residues constitute the major carbohydrate moiety in the EPS. In parallel, we synthesized, expressed, purified, and characterized 15 putative depolymerase enzymes by a variety of assays against static, dynamic, and mixed biofilm conditions. At least eight enzymes displayed significant anti-biofilm activity and three (NagZ, DspNW, and Betty) were advanced to animal safety testing.