Resistance monitoring of Plutella xylostella (L.) (Lepidoptera: Plutellidae) to risk-reduced insecticides and cross resistance to spinetoram

摘要

The diamondback moth, Plutella xylostella L. (Lepidoptera: Plutellidae) is the major pest of the Brassicaceae family. In the Agreste region of Pernambuco (Brazil), a Brassica producing center, P. xylostella is under continuous selection pressure by insecticides leading to increased frequency of resistance genes in this species. The objective of the present study was to monitor the resistance of P. xylostella populations to spinosad, chlorfenapyr, and chlorantraniliprole as well as the susceptibility to spinetoram in the Agreste region of Pernambuco (Brazil). Concentration-response bioassays were performed with spinosad, chlorfenapyr and spinetoram to estimate the LC values. Furthermore, a concentration corresponding to label dose for registered products (chlorantraniliprole, spinosad, and chlorfenapyr) and diagnostic concentration for chlorantraniliprole and spinosad were used. The LC50 to spinosad varied from 0.017 (Recife) to 3.64 (Bezerros II) mg a. i./L (RR50 varying up to similar to 200-fold), while for spinetoram, they varied from 0.0013 (Alegre) to 0.198 (Bezerros II) mg a. i./L (RR50 varying up to similar to 150-fold). The LC50 for chlorfenapyr ranged from 0.43 (Recife) to 42.23 (Bezerros II) mg a. i./L (RR50 varying up to similar to 100-fold). Plutella xylostella populations developed resistance to chlorfenapyr and spinosad. Apparently, there was no alteration of the resistance levels to chlorantraniliprole in the field. Cross-resistance between spinosad and spinetoram (RR50 similar to 150-fold) as well as between the spinosyns and chlorfenapyr was observed. The field populations were susceptible to concentration corresponding to label doses of spinosad (mortality > 80 %) and chlorfenapyr (mortality > 80 %), but a considerable loss of susceptibility to both insecticides was observed. Immediate attention to correct rotation of products is necessary to delay development of resistance in this pest.