Direct organogenesis and plant regeneration from petiole explant of male himalayan poplar (Populus ciliata Wall.).

摘要

Plant regeneration studies were carried out on male plant of Himalayan poplar (Populus ciliata Wall.) to standardize a protocol for high frequency plant regeneration. Young and tender petioles were used as explant, which were obtained from the nursery raised plants. High frequency shoot regeneration via direct organogenesis was obtained on Murashige and Skoog's medium supplemented with 1.50 mg/l Kinetin and 0.10 mg/l Indole-3-acetic acid. High percentage root regeneration in adventitious shoots was obtained on MS medium supplemented with 0.10 mg/l Indole-3-acetic acid. The experimental results described on regeneration of whole plantlets from the explant indicate that young and tender petiole explant is as important as the correct plant growth regulator's balance for high frequency plant regeneration. Himalayan poplar plantlets were able to regenerate within two months and young plantlets were transferred to pots for hardening. Hardening procedure for tissue culture raised plantlets was standardized.