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Progress In Breeding Sclerotinia Blight-Resistant Runner-Type Peanut

机译:抗核疫病转轮型花生育种研究进展

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Field screening tests were conducted in 1991, 1992, and 1993 to identify Sclerotinia blight-resistant runner-type peanuts. Selections were made from three populations, "backcross to runner," "backcross to Tamspan 90," and "single cross." Runner parents were chosen for agronomic qualities and resistance to other pathogens. Resistant (spanish) parents used were cultivar Tamspan 90 and TxAG-5 germplasm. Families within populations were planted as single row 3 x 0.9-m plots. Repeated plant-by-plant inspections (PBPI) were made for assessment of sequence of infection and subsequent calculation of an area under the disease progress curve (AUDPC). Most families in the backcross to Tamspan 90 population consistently had a disease reaction close to or lowerthan Tamspan 90 but also retained undesirable plant forms (i.e., upright). By 1993, the F_(2:5) backcross to runner population had the highest percentage of runner-type families (88%) and a mean disease reaction close to Tamspan 90. Whole plot evaluationof Sclerotinia blight severity was compared with PBPI for effectiveness on a segregating population containing a total of 298 F_(4:7) single cross and F_(2:5) backcross entries. The whole plot system used a scale of 0 = no visible plot infection to 5 >80% infection in the plot. Correlation between area under disease progress curve values of both methods was high (>.85). Remnant seed of selections screened for resistance in the greenhouse were planted in the field and evaluated for resistance. Chi-square test for independence indicated a significant (a = .012) relationship between greenhouse and field performance. Field screening and limited greenhouse screening appears to be an effective way to develop resistant runner-type peanut lines.
机译:1991年,1992年和1993年进行了田间筛选试验,以鉴定抗核盘菌枯萎型转轮型花生。从三个种群中进行选择,即“与跑步者的回交”,“与坦斯潘90的回交”和“单交叉”。选择赛跑父母是因为其农艺品质和对其他病原体的抵抗力。使用的抗性(西班牙)亲本是Tamspan 90品种和TxAG-5种质。人口中的家庭被种植成单行3 x 0.9米的地块。进行了逐厂重复检查(PBPI),以评估感染顺序并随后计算疾病进展曲线下的面积(AUDPC)。回交至Tamspan 90种群的大多数家庭始终具有接近或低于Tamspan 90的疾病反应,但也保留了不良的植物形态(即直立)。到1993年,F_(2:5)与转基因人群的回交率最高,达到了转基因类型家族的百分比(88%),平均疾病反应接近Tamspan90。对菌核病疫情严重程度的全小区评估与PBPI的有效性进行了比较。包含总共298个F_(4:7)单杂交和F_(2:5)回交条目的隔离种群。整个地块系统的等级为0 =无可见地块感染,地块中的5> 80%感染。两种方法的疾病进展曲线值下面积之间的相关性很高(> .85)。在温室中筛选出用于抗性筛选的选择的剩余种子,并在田间种植并评估抗性。卡方检验的独立性表明,温室效应与田间表现之间存在显着的(a = .012)关系。田间筛选和有限温室筛选似乎是开发抗性转轮型花生株系的有效方法。

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