Pancreatic ischemia/reperfusion injury: impact of different preservation temperatures.

摘要

OBJECTIVES: Pancreatic ischemia/reperfusion injury (IRI) can influence the results after transplantation. Temperature during ischemia can affect IRI. A temperature of 4 degrees C is assumed as optimal for graft preservation. There are no data about the impact of different ischemia temperatures in pancreatic IRI. METHODS: Ischemia/reperfusion injury was induced in pancreatic tail segments (2-hour ischemia, 2-hour reperfusion), with rats (7/group) without ischemia served as control. Animals were randomized to the different experimental groups. To achieve the desired temperature (4, 18, or 37 degrees C and 37 degrees C control), pancreatic tail segments were superfused with temperated saline. After reperfusion, microcirculation was observed by intravital fluorescence microscopy. Functional capillary density (FCD), leukocyte adherence in post-capillary venules, and histological damage were analyzed. RESULTS: In IRI groups, decrease of FCD 1 and 2 hours after reperfusion compared with baseline measurements was significant. Functional capillary density in 4 degrees C was better as compared with 18 and 37 degrees C after reperfusion. Lower adherent leukocytes were seen in 4 and 18 degrees C, compared with 37 degrees C and also to CO. In 4 degrees C, histological damage was lower as compared with 18 and 37 degrees C. CONCLUSIONS: We could demonstrate that also in pancreatic IRI, tissue injury is temperature dependent. Compared with 37 degrees C, although a protective effect is established already at 18 degrees C, more protection is achieved with storage at 4 degrees C. Our data suggest that 4 degrees C has the best protective effect on pancreatic IRI.