Simultaneous determination of triterpene saponins in Ginseng drugs by high-performance liquid chromatography

摘要

A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mm K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signaloise greater than or equal to3) were 0.1 mug for ginsenosides Rb-1, Rc, Rd, Re and Rg(1), chikusetsusaponin III, and notoginsenoside R-2, 0.2 mug for gisenoside R-0 and chikusetsusaponin IVa, 0.3 mug for chikusetsusaponin IV, and 3 mug for majonoside R-2. The calibration curve of each saponin had a correlation coefficient close to 1. Intraand interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved.