The zymooram pattern of the new type of SBTi-A2 of seed protein and the crossing with other alleles

摘要

We found one new germplasm which trypsin inhibitor zymogram pattern is different from Ti~a, Ti~b, and Ti~c from cultivated soybeans (Glycine max) in China in 1991. During 1992 and 1994 we grew it in Jilin Academy of Agricultural Sciences (Gongzhuling,Jilin Province, China), in greenhouse when in winter and in field when in summer. It was planted six times in 3 years. In the meantime we planted it in Jilin Province in 1992, and planted it in Shandong Province in 1993. 8 shares of seeds were gatheredand detected by electrophoresis. The result coincided to that in 1991 completely. Tthis showed that the new type (Tix) has stable heredity. We purified Ti~x from these seeds for biochemical experiment. Reciprocal crossing was done using this variety as parent with the varieties of Ti~a, Ti~b, Ti~c, titi (L81-4590). We reported the purification of Ti~x and the electrophoretic result of FI seeds.