Clone pBLT65 is Associated with Soybean Cyst Nematode (SCN) Race 1 Resistance in J87-233

摘要

The germplasm soybean line J87-233 (maturity group V) is resistant to soybean cyst nematode (SCN) races 1, 2, 3 and 5 with moderate resistance to race 14, as well as root knot nematode (Young, 1993). It derives SCN resistance from three primitive soybean plant introductions: PI 88788, PI 90763, and Peking. Hutcheson is a high yielding maturity group V cultivar that is susceptible to SCN (Buss et al. 1988). The F_2:3 progeny population of the cross J87-233 x Hutcheson has been used to characterize themolecular profile of SCN resistance in J87-233. DNA molecular markers specific for SCN resistance have been identified in several primitive sources widely used in soybean breeding programs (Concibido et al. 1994; Mahalingam and Skorupska 1995; Webb et al. 1995). Two cDNA clones, pBLT24 and pBLT65, gave polymorphisms with TaqI and Haein, respectively, that have been shown to be very tightly linked to the i locus conferring self dark seed coat color (Weisemann et al. 1992). A quantitative trait locus for SCN race 3 resistance was localized in close proximity to the i locus (Mahalingam and Skorupska, 1995; Webb et al. 1995).