Raman Spectroscopy-Based Multivariate Statistical Analysis Reveals the Molecular Mechanism of K562 Cell Apoptosis Induced by Adriamycin

摘要

Based on the combination of Raman spectroscopy with principal component analysis and hierarchical cluster analysis, the molecular mechanism of K562 cell apoptosis induced by Adriamycin in physiological conditions is presented. The obtained results reveal that DNA of K562 cells treated with Adriamycin is lowered greatly, indicating that the damage of the DNA of K562 cells is indeed the main molecular mechanism of K562 cell apoptosis induced by Adriamycin. Specially, by combining principal component analysis and hierarchical cluster analysis, the statistical difference between Raman spectra of different cell types can be revealed effectively. Importantly, this kind of Raman spectroscopy-based multivariate statistical analysis will supply a useful tool for the molecular mechanism detection of cell behavior in physiological conditions.